bv2 cell line  (Keygen Biotech)

Journal: Journal of Biomedical Research

Article Title: HDGF derived from Müller cells enhances the activation of microglia in diabetic retinopathy

doi: 10.7555/JBR.38.20240386

Figure Lengend Snippet: HDGF promoted microglia activation and IL-1β secretion. A: Analysis of a published list of target genes of HDGF integrated with mouse retinal single-cell RNA sequencing data, indicating predominant target cell types. The violin plot shows expression of the target gene Itgb2 in microglia from control and streptozotocin (STZ)-induced diabetic mouse retinas. The microglia cluster is indicated. B: Enzyme-linked immunosorbent assay measuring IL-1β secretion in the supernatant of BV2 microglial cells. Cells were treated under low-glucose (LG, 1 g/L) or high-glucose (HG, 5 g/L) conditions for 48 h, with or without recombinant HDGF (rHDGF) and an HDGF-neutralizing antibody (anti-HDGF) ( n = 3). C: Quantitative PCR analysis of Itgb2 mRNA expression in BV2 cells treated as indicated ( n = 3). D: Representative immunofluorescence images (left) and quantification (right) of ITGB2-positive BV2 cells following the treatments. Nuclei were counterstained with DAPI. Quantitative analysis of ITGB2-positive cells treated as indicated ( n = 3). E: Representative immunofluorescence images (left) and quantification (right) of CD68-positive BV2 cells following the treatments. Nuclei were counterstained with DAPI. Quantitative analysis of CD68-positive cells treated as indicated ( n = 3). F: Feature plot from retinal scRNA-seq data showing specific Itgb2 expression within the microglia cluster (outlined). G: Western blotting analysis of ITGB2 protein expression in BV2 microglial cells and primary Müller cells. α-Tubulin served as the loading control. Quantitative protein levels of ITGB2 in BV2 and Müller cells ( n = 3 per group). Data are presented as mean ± standard deviation. Statistical significance was determined by one-way analysis of variance (ANOVA; B–E) or paired Student's t -test (G). * P < 0.05; ** P < 0.01; *** P < 0.001. Abbreviations: HDGF, hepatoma-derived growth factor; ITGB2, integrin beta 2.

Article Snippet: The immortalized murine microglial cell line (BV2) (Cat. #CRL-2469) was purchased from the American Type Culture Collection and cultured in DMEM/F12 medium (Gibco) supplemented with 10% FBS (Invitrogen), 1% glutamine (Invitrogen), and streptomycin (100 U/mL; Invitrogen) at 37 °C, 5% CO 2 .

Techniques: Activation Assay, RNA Sequencing, Expressing, Control, Enzyme-linked Immunosorbent Assay, Recombinant, Real-time Polymerase Chain Reaction, Immunofluorescence, Western Blot, Standard Deviation, Derivative Assay

Journal: Journal of Biomedical Research

Article Title: HDGF derived from Müller cells enhances the activation of microglia in diabetic retinopathy

doi: 10.7555/JBR.38.20240386

Figure Lengend Snippet: High glucose upregulated the expression of HDGF receptor in microglia. A: Western blotting analysis of HDGF protein expression in BV2 microglial cells cultured under low-glucose (LG, 1 g/L) or high-glucose (HG, 5 g/L) conditions for 48 h. α-Tubulin served as the loading control. Quantitative protein levels of ITGB2 in BV2 and Müller cells ( n = 3 per group). B: Representative immunofluorescence images of retinal cryosections from control and streptozotocin (STZ)-induced diabetic mice, co-stained for HDGF (red) and the microglial marker IBA1 (green). Nuclei were counterstained with DAPI (blue). Assessment of immunofluorescence colocalization of HDGF and IBA1 in the two experimental groups (left, control; right, STZ). C: Single-cell RNA sequencing analysis of control and STZ-diabetic mouse retinas. UMAP plot showing the expression of Ncl (the gene encoding nucleolin) across all cells (upper). The microglia cluster is indicated. Dot plot illustrating the relative expression level and proportion of Ncl -expressing cells in microglia in the control and STZ groups (lower). D: Representative immunofluorescence images (left) and quantification of mean fluorescence intensity (MFI, right) for NCL (green) in BV2 cells cultured under LG or HG conditions for 48 h. Nuclei were counterstained with DAPI (blue). n = 3. Data are presented as mean ± standard deviation. Statistical significance was determined by the paired Student's t -test. * P < 0.05. Abbreviations: HDGF, hepatoma-derived growth factor; IBA1, ionized calcium-binding adaptor molecule 1; ns, not significant; STZ, streptozotocin.

Article Snippet: The immortalized murine microglial cell line (BV2) (Cat. #CRL-2469) was purchased from the American Type Culture Collection and cultured in DMEM/F12 medium (Gibco) supplemented with 10% FBS (Invitrogen), 1% glutamine (Invitrogen), and streptomycin (100 U/mL; Invitrogen) at 37 °C, 5% CO 2 .

Techniques: Expressing, Western Blot, Cell Culture, Control, Immunofluorescence, Staining, Marker, RNA Sequencing, Fluorescence, Standard Deviation, Derivative Assay, Binding Assay

Journal: bioRxiv

Article Title: Olfactory proteomics reveals the capacity of the HDAC1 inhibitor pyroxamide to halt the α-synuclein preformed fibrils-induced damage in nasal epithelial, microglial and dopaminergic neuronal cell lines

doi: 10.1101/2025.10.02.679944

Figure Lengend Snippet: In vitro experiments in dopaminergic MN9D cells representing the survival effect of α-syn PFFs after 6h of pyroxamide pre-treatment (A). Survival effect of α-syn PFFs after 3 and 6 hours of pyroxamide pre-treatment in olfactory epithelial T9243 cells (B). Survival effect of α-syn PFFs after 3 hours of pyroxamide pre-treatment in microglial BV2 cells upon α-syn PFFs-induced damage (C). Data were analyzed using one-way ANOVA. * indicates p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Similar conditions were used for the Dopaminergic Neuronal Cell Line (MN9D; Merck Millipore, SCC281), and the Mouse Microglial Cell Line (BV2; Cytion, 305156), seeded at 1 × 10^4 cells/well in 96-well plates.

Techniques: In Vitro

Journal: bioRxiv

Article Title: Olfactory proteomics reveals the capacity of the HDAC1 inhibitor pyroxamide to halt the α-synuclein preformed fibrils-induced damage in nasal epithelial, microglial and dopaminergic neuronal cell lines

doi: 10.1101/2025.10.02.679944

Figure Lengend Snippet: Survival analysis associated to pyroxamide pre-treatment 3 hours in dopaminergic neuronal (A) and microglial cells (B) upon oxidative stress conditions. Data were analyzed using one-way ANOVA. * indicates p < 0.05; ** p < 0.01.

Article Snippet: Similar conditions were used for the Dopaminergic Neuronal Cell Line (MN9D; Merck Millipore, SCC281), and the Mouse Microglial Cell Line (BV2; Cytion, 305156), seeded at 1 × 10^4 cells/well in 96-well plates.

Techniques:

Journal: Bioactive Materials

Article Title: Dual-pathway targeted therapy for Parkinson's disease: Biomimetic nanosomes inhibit ferroptosis and pyroptosis through NLRP3 inflammasome regulation

doi: 10.1016/j.bioactmat.2025.06.033

Figure Lengend Snippet: Characterization of BM@GA-Se nanosomes. (a) TEM image of GA-Se NPs. (b) TEM image of BM@GA-Se nanosomes. (c) DLS of GA-Se NPs and BM@GA-Se nanosomes. (d) Zeta potential of GA-Se NPs, BV2 cell and BM@GA-Se nanosomes. (e) Protein bands of BV2 cell membrane and BM@GA-Se nanosomes. (f) XRD results of GA-Se NPs, gallic acid, and Na 2 SeO 3 . (g) XPS survey of GA-Se NPs. (h) Peak differentiation of C1s. (i) Peak differentiation of O1s. (j) Peak differentiation of Se3d. (k) FT-IR stretching bonds of GA-Se NPs, gallic acid, and Na 2 SeO 3 . (l) TGA curves of GA-Se NPs, BM@GA-Se NPs, gallic acid, and Na 2 SeO 3 .

Article Snippet: BV2 cell line was obtained from Procell (Wuhan, China) and cultivated in corresponding specialized culture medium.

Techniques: Zeta Potential Analyzer, Membrane

Journal: Frontiers in Pharmacology

Article Title: Comprehensive profiling of Rhodiola rosea roots and corresponding products: phytochemical insights and modulation of neuroinflammation in BV2 microglial cell model

doi: 10.3389/fphar.2025.1608767

Figure Lengend Snippet: The influence of tested extracts (50 μg/mL) and rosavin (0.1–5 μM) on TNF-α secretion by LPS-stimulated microglia BV2. Data from three separate experiments assayed in duplicate are expressed as mean ± SEM. Dexamethasone (DEX, 20 μM) was used as a positive control. Absorbance values for all samples were expressed as percentages relative to the LPS-stimulated control (KST), which was set at 100%. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001 vs. stimulated control (KST), # statistically significant ( p < 0.001) vs. non-stimulated control (KNST).

Article Snippet: The immortalised murine microglial cell line BV2 (passages 1–4) was purchased from DSMZ–German Collection of Microorganisms and Cell Cultures GmbH.

Techniques: Positive Control, Control

Journal: Frontiers in Pharmacology

Article Title: Comprehensive profiling of Rhodiola rosea roots and corresponding products: phytochemical insights and modulation of neuroinflammation in BV2 microglial cell model

doi: 10.3389/fphar.2025.1608767

Figure Lengend Snippet: The influence of tested extracts (50 μg/mL) and rosavin (0.1–5 μM) on IL-6 secretion by LPS-stimulated microglia BV2. Data from three separate experiments assayed in duplicate are expressed as mean ± SEM. Dexamethasone (DEX, 20 μM) was used as a positive control. Absorbance values for all samples were expressed as percentages relative to the LPS-stimulated control (KST), which was set at 100%. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001 vs. stimulated control (KST), # statistically significant ( p < 0.001) vs. non-stimulated control (KNST).

Article Snippet: The immortalised murine microglial cell line BV2 (passages 1–4) was purchased from DSMZ–German Collection of Microorganisms and Cell Cultures GmbH.

Techniques: Positive Control, Control